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Image Search Results
Journal: Molecular Cell
Article Title: Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition
doi: 10.1016/j.molcel.2019.03.041
Figure Lengend Snippet:
Article Snippet: All mouse ESC lines e.g., e14tg2a, IDG3.2 ( ) and tamoxifen inducible (concentration of 1ug/mL or ∼2.5uM of (Z)-4-Hydroxytamoxifen, H7904, Sigma-Aldrich) Tardbp conditional knockout (termed cTdp-43 KO ( )) were maintained on 0.1% gelatin-coated plates in 1:1 Neurobasal (21103049) DMEM (11320033) medium containing N2 (17502048) and B27 (17504044) supplements, 1% Glutamax (35050), 1% nonessential amino acids (1140050), 0.1 mM 2-mercaptoethanol (31350-010) (all Thermo Fisher Scientific), 1 μM
Techniques: Recombinant, Produced, Gentle, Knock-Out, Reverse Transcription, TaqMan Assay, Labeling, Staining, Flow Cytometry, Western Blot, Clarification Assay, Membrane, Sample Prep, Sequencing, Derivative Assay, Next-Generation Sequencing, Expressing, Fractionation, Activation Assay, Nucleic Acid Electrophoresis, CRISPR, Plasmid Preparation, Modification, shRNA, Software